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KMID : 0545119940040020155
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Journal of Microbiology and Biotechnology
1994 Volume.4 No. 2 p.155 ~ p.158
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Expression of lac and gal operons in Zymomonas mobilis
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CHO, DONG-WUK
ROGERS, PETER L./DELANEY, STEPHEN F.
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Abstract
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Two Zymomonas mobilis strains (ZM63 and ZM6307), containing both lactose and galactose operons, were constructed. β-Galactosidase and galactokinase assays indicated that both operons were expressed in both strains. The transport systems available for lactose uptake by Zymomonas mobilis were investigated using ^14C-labelled lactose. After the outer membrane, which was considered to be a possible barrier to lactose uptake, was disrupted by treatment with EDTA and Ca^2+ ions, some increase in lactose uptake was observed in ZM6306 (lac^+) and ZM6307 (lac^+ gal^+), but not in the parent, ZM6. This suggested that the outer membrane of Zymomonas mobilis acts as a barrier to lactose uptake to some degree, and also that the lactose permease is operational in Zymomonas mobilis.
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